SKU: 19041245839

Porcine CCL20/MIP3a ELISA Kit

Sale price$343.80 Regular price$382.00
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Ships within 48 hours · Estimated delivery Jul 14 - Jul 19

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Description

Porcine CCL20/MIP3a ELISA KitThe Porcine CCL20 MIP3a ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Pig MIP3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Pig MIP3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only

The Porcine CCL20/MIP3a ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MIP3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MIP3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MIP3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MIP3a in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 19041245839

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