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Description
Human IL12A (Interleukin-12 Subunit Alpha) ELISA KitThe Human (IL12A) Interleukin 12 Subunit Alpha ELISA Kit measures Interleukin 12 Subunit Alpha in samples. The plate has been pre coated with Human IL12A antibody. IL12A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL12A Antibody is added and binds to IL12A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL12A antibody. After incubation unbound Streptavidin HRP is
The Human (IL12A) Interleukin-12 Subunit Alpha ELISA Kit measures Interleukin-12 Subunit Alpha in samples. The plate has been pre-coated with Human IL12A antibody. IL12A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL12A Antibody is added and binds to IL12A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL12A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL12A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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