SKU: 2443349676

Canine IFN-Alpha (Interferon Alpha) ELISA Kit

Sale price$292.50 Regular price$325.00
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Ships within 48 hours · Estimated delivery Jul 12 - Jul 17

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Description

Canine IFN-Alpha (Interferon Alpha) ELISA KitThe Canine IFN Alpha (Interferon Alpha) ELISA Kit measures IFN Alpha in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IFN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Canine IFN-Alpha (Interferon Alpha) ELISA Kit measures IFN-Alpha in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IFN-α antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IFN-α bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IFN-α in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 2443349676

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