SKU: 63395618392

Porcine OBESTATIN ELISA Kit

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Description

Porcine OBESTATIN ELISA KitThe Porcine OBESTATIN ELISA Kit measures Obestatin in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with OB. OB in the sample or standard competes with a fixed amount of biotin labeled OB for sites on a pre coated antibody specific to OB. Free components are washed off. After adding HRP

The Porcine OBESTATIN ELISA Kit measures Obestatin in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with OB. OB in the sample or standard competes with a fixed amount of biotin-labeled OB for sites on a pre-coated antibody specific to OB. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of OB in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 63395618392

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Chris
Lexington, US
★★★★★ 3
Nice Artwork
Format: Paperback
This is a very colorful book with nice artwork. A fun book to look at for young kids. I received a free copy of this book via Booksprout and am voluntarily leaving a review.
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Reviewed in the United States on February 22, 2021
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Amazon Customer
Cuba, US
★★★★★ 5
Funny story for kids.
Format: Paperback
My son who is 7 years old can’t get enough of this book! It a funny story that any child in his age bracket will enjoy. It was a perfect addition to his Easter basket.
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Reviewed in the United States on May 8, 2021
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Kathryn Reynolds
Louisville, US
★★★★★ 5
Quality
Format: Hardcover
Grandson loves books
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Reviewed in the United States on May 15, 2026
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Lindsay L Huntington
Cuba, US
★★★★★ 5
A book every teacher/parent should read!
Format: Hardcover
I LOVE this book because the "bad seed" reminds me of my son. I don't believe there are any "bad kids" in the world but I'm sure kids look at themselves as bad if that is all they ever hear. I love how this story gives a sympathetic backstory on how he got "so bad" and how he is trying to do better and change his bad ways. Even though my son isn't perfect, he sure does try to do good and his behavior improves when people notice the POSITIVE instead of the negative. I absolutely love the last lines of the book where the other seeds are gossiping (another lesson) saying, "Hey! There goes that bad seed," and another seed sticks up for him and says, "Actually, he isn't that bad anymore." Then the "bad seed" turns around with a smile on his face and says, "I heard that." Moral of the story.....children are always listening. Focus on the positive rather than the negative and a child's self-talk and behavior will drastically improve. My son's 2nd grade year was so negative and he was always getting into trouble. I rarely heard the good and neither did my son. His 3rd grade year was amazing. The teacher really focuses on pointing out his good choices and what a difference that made in my son's attitude toward school. Thank you Jory John for writing this book. I also love the book, "I Love You Already" which teaches readers about friendship, love, patience, loyalty and forgiveness. :)
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Reviewed in the United States on October 2, 2018
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Patty Rosebourgh
Bozeman, US
★★★★★ 5
Kids learn to keep going
Format: Hardcover
Used for read aloud
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Reviewed in the United States on February 18, 2026

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