SKU: 89238216667

Human ASIP (Agouti-signaling protein) QuickTest ELISA Kit

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Description

Human ASIP (Agouti-signaling protein) QuickTest ELISA KitThe Human ASIP (Agouti signaling protein) QuickTest ELISA Kit measures ASIP in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples

The Human ASIP (Agouti-signaling protein) QuickTest ELISA Kit measures ASIP in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains ASIP, a capture antibody-ASIP-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ASIP in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 89238216667

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Amanda
Massapequa, US
★★★★★ 5
Perfect
Color: Blue
I am fostering a Husky/Black and Tan Coon dog. We have had her since early February. She has chewed up a lot of my shoes. She destroyed my other dogs stuffed squeaky toys. So I decided to buy her this ball. She reminds me of Linus from Charlie Brown. She wakes up with this ball and falls asleep with it. I’ve not had another shoe fatality. This ball is her most loved possession. It is pretty tough. Now She did kill the squeaker within the first few hours (which trust me I am GOOD with that! I’ve never put treats in it. The rope is still attached. After this review I am going to buy her another as a back up!
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Reviewed in the United States on April 14, 2023
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JLF
Belleville, US
★★★★★ 5
Favorite fetch
We have a 70 lb Aussie and a 13 lb Havanese who compete with each other to fetch toys. This hedgehog is their favorite, largely due to the loud assertive squeak. Our big dog is a chewer and has a history of getting to any squeaker in a toy, once nearly choking when successfully removing one from a smaller toy. She still tries to chew on this toy, going straight for the nose but has not yet gotten to the squeaker which is large enough that I don't believe would be a choking hazard if she did get to it. No soft toy is indestructible, but compared to other plush toys, this is more durable. Once the nose has been chewed off, I have simply hand stitched the hole closed and from then on she's no longer interested in chewing through it. Have run through the wash and comes out fine. When one eventually wears out I order a replacement and the 2 puppies compete once again over ownership.
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Reviewed in the United States on January 17, 2023
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Larkus
Carnegie, US
★★★★★ 5
Holding up to my teething 5 month old puppy
All toys are are hard to review as it comes down to the pet. But for what it's worth, this seems well made and is holding up to our teething puppy!
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Reviewed in the United States on March 27, 2026
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Debbie C.
Carnegie, US
★★★★★ 5
Best toy
My dog's favorite hedgehogs. I ordered 3 at a time.
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Reviewed in the United States on January 8, 2026
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Dorothy
Dallas, US
★★★★★ 5
Well made so adorable
My Daisy loves well made so cute love the sound not to loud. I would definitely recommend
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Reviewed in the United States on October 16, 2025

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